Composite
Pc-PQSR

Part:BBa_K1157019

Designed by: Lin Ang Chieh   Group: iGEM13_NTU-Taida   (2013-09-15)

Pc-PQSR

This is a sub-part of quorum sensing molecule biosensor 2-heptyl-3-hydroxy-4(1H)-quinolone sensed by PQS receptor. The constitutive promoter expresses PQS receptor. In the final construct (this part plus BBa_K1157017), if the PQS receptor(PQSR) binds with 2-heptyl-3-hydroxy-4(1H)-quinolone, it will be able to regulate its promoter sequence resulting in expression of reporter GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 525
    Illegal BamHI site found at 141
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 752
    Illegal NgoMIV site found at 973
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 709


[edit]
Categories
Parameters
None